During polymerization, nucleotides are added in which direction?

Study for the DNA Structure, Replication, Transcription and Translation Test with flashcards and multiple choice questions. Each question offers hints and explanations. Prepare thoroughly and excel in your exam!

Multiple Choice

During polymerization, nucleotides are added in which direction?

Explanation:
Nucleotides are added to the free 3' end of the growing strand, so the polymer becomes longer at the 3' end and synthesis proceeds in the 5' to 3' direction. This happens because DNA (or RNA) polymerase uses the 3' hydroxyl of the last nucleotide to attack the incoming nucleotide’s 5' phosphate, forming a new phosphodiester bond and releasing pyrophosphate. As a result, the new strand is built in the 5' to 3' direction while the template is read 3' to 5'.

Nucleotides are added to the free 3' end of the growing strand, so the polymer becomes longer at the 3' end and synthesis proceeds in the 5' to 3' direction. This happens because DNA (or RNA) polymerase uses the 3' hydroxyl of the last nucleotide to attack the incoming nucleotide’s 5' phosphate, forming a new phosphodiester bond and releasing pyrophosphate. As a result, the new strand is built in the 5' to 3' direction while the template is read 3' to 5'.

More Multiple Choice Questions
Subscribe

Get the latest from Passetra

You can unsubscribe at any time. Read our privacy policy